Comparison of Corneal Epithelial and Stromal Thickness Distributions between Eyes with Keratoconus and Healthy Eyes with Corneal Astigmatism ≥2.0 D

PURPOSE To identify corneal epithelial- and stromal-thickness distribution patterns in keratoconus using spectral-domain optical coherence tomography (SD-OCT). PATIENTS AND METHODS We analyzed SD-OCT findings in 20 confirmed cases of keratoconus (group 1) and in 20 healthy subjects with corneal astigmatism ≥ 2 D (group 2). Epithelial and stromal thicknesses were measured at 11 strategic locations along the steepest and flattest meridians, previously located by corneal topography. Vertical mirrored symmetry superimposition was used in the statistical analysis. RESULTS The mean maximum keratometry measurements in groups 1 and 2 were 47.9 ± 2.9 D (range, 41.8-52.8) and 45.6 ± 1.1 D (range, 42.3-47.5), respectively, with mean corneal cylinders of 3.3 ± 2.2 D (range, 0.5-9.5) and 3.6 ± 1.2 D (range, 2.0-6.4), respectively. The mean epithelial thickness along the steepest meridian in group 1 was the lowest (37.4 ± 4.4 µm) at 1.2 mm inferotemporally and the highest (59.3 ± 4.4 µm) at 1.4 mm supranasally from the corneal vertex. There was only a small deviation in thickness along the steepest meridian in group 2, as well as along the flattest meridians in both groups. The stromal thickness distribution in the two groups was similar to the epithelial, while the stromal thickness was generally lower in group 1 than in group 2. CONCLUSIONS SD-OCT provides details about the distribution of corneal epithelial and stromal thicknesses. The epithelium and stroma in keratoconic eyes were thinner inferotemporally and thicker supranasally compared with control eyes. The distribution pattern was more distinct in epithelium than in stroma. This finding may help improve the early diagnosis of keratoconus. TRIAL REGISTRATION ClinicalTrials.gov NCT02023619.